CCL-7 LLC-MK2 恒河猴腎細胞
ATCC® Number:  CCL-7?      
Designations:  LLC-MK2 Original 
Depositors:   RN Hull 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Macaca mulatta (monkey, rhesus) 
Morphology: epithelial

 
Source: Organ: kidney
Disease: normal
Cellular Products: plasminogen activator 
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Virus Susceptibility: poliovirus 1; coxsackievirus B4; bovine viral diarrhea (BVD) virus
Reverse Transcript: negative 
Age:  
Comments: The serum used in the medium should be pre-tested for good quality.
Some lots of horse serum do support satisfactory growth.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth.
Propagation:  ATCC complete growth medium: Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1%
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Preservation:  Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2040
References: 22310: . . Anat. Rec. 124: 490, 1956.
26049: Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901
26065: Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902